625 Pulsed Electromagnetic Field and tissue repair: an experimental study on chronic wounds’ fibroblasts

Extremely low frequency electromagnetic fields (ELF-EMFs) are non-ionizing, low-energy, fields, capable of inducing biological effects such as cell migration, proliferation and differentiation, cytokine growth factors expression, NO signaling alteration. In vitro experiments have shown an interaction ELF-EMFs with all the phases wound healing. This in knowledge could be applied clinical practice to chronic wounds, which result a deregulation Our study was conducted on Human Dermal Fibroblasts isolated from 20 patients venous ulcers. One punch collected ulcer, while another healthy skin site. were cultured DMEM tissue culture plate. After 24h cells incubated presence Pulsed field (PEMF). addition, parallel performed without PEMF exposure. Cells exposed for 3 different experimental times. For each exposure condition, fibroblasts’ evaluated using images acquired inverted microscope. The cell’s adhesion capacity monitored at time 0 after 6, 24, 48, 72h. condition that better improved confluence selected isolating total RNA fibroblasts. retro transcribed analyzed detect relative expression TGFβ, TNFα, IL6, IL1β, INTβ1, MMP9, MMP2. As result, reduced gene ulcerative fibroblasts comparison unexposed To date, there no vivo studies demonstrating treatment, only ex studies, been cells. is first compare PEMS ulcer’s same patients.